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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Microbiology | 15-20% | - Specimen collection and culture techniques - Mycology, parasitology, and virology - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility |
| Immunology and Serology | 5-10% | - Immune system principles and components - Infectious disease serology - Immunologic testing methodologies - Autoimmune and immunodeficiency disorders |
| Laboratory Operations | 5-10% | - Laboratory mathematics and instrumentation - Quality control and quality assurance - Pre-analytical, analytical, and post-analytical processes - Laboratory safety and regulatory compliance |
| Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Antibody detection and identification - Transfusion reactions and adverse events - Compatibility testing - Blood group systems and antigens |
| Urinalysis and Other Body Fluids | 5-10% | - Semen and amniotic fluid testing - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Physical, chemical, and microscopic analysis |
| Hematology | 20-25% | - White blood cell morphology and diseases - Hemostasis and coagulation testing - Red blood cell physiology and disorders - Routine and automated hematology procedures |
| Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question #1
The microscopic features shown here represent Scopulariopsis species. In most instances, particularly if a patient does not have underlying immunologic or hematologic disease, Scopulariopsis species should be considered a contaminant when recovered from a sputum specimen. However, if there is clinical or X-ray evidence of mycotic pulmonary infection, additional daily induced sputum specimens should be obtained.
If Scopulariopsis species or any other hyaline mold is recovered from two or more successive specimens, its potential as a pathogenic agent should be considered. Scopulariopsis species have been reported as the agents of pulmonary fungus ball infections in patients with preexistent cavities and as a cause of pneumonia in patients with leukemia.
Invasive pulmonary disease by this agent has not been reported.
The fungus illustrated in this photomicrograph was recovered from an induced sputum specimen from a 74 year old man with chronic obstructive pulmonary disease. This isolate is most likely:
A. The cause of chronic bronchitis
B. The cause of invasive pulmonary disease
C. A contaminant
D. The cause of allergic bronchopulmonary disease
Question #2
Interferon gamma serves various purposes in immunologic response, including the promotion of natural killer cell activity as well as decreased viral replication by using enzymes to decrease protein replication, both viral or host, inside of the cell.
The lymphokine most important for increased natural killer cell activity and decreased viral replication in cells is:
A. Interleukin 5
B. Interferon gamma
C. Interleukin 4
D. Interleukin 3
Question #3
Standard precautions should be followed:
A. with all patients, at all times
B. when a patient is known to have hepatitis B
C. if a patient is in isolation
D. when a patient is known to be HIV positive
Question #4
The most common specimen analyzed in the hematology section is:
A. serum.
B. urine.
C. plasma.
D. whole blood.
Question #5
As magnification INCREASES, the field of view will...
A. decrease (get smaller)
B. stay the same
C. increase (get bigger)
Solutions:
| Question #1 Correct Answer: C | Question #2 Correct Answer: B | Question #3 Correct Answer: A | Question #4 Correct Answer: D | Question #5 Correct Answer: A |


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